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BiologistBinding Minds Inc. (Certified Disability Owned Business Enterprise) • Boston, MA, United States
Biologist
Binding Minds Inc. (Certified Disability Owned Business Enterprise) • Boston, MA, United States
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job_description.job_card.job_descriptionBS or MS in Stem Cell Biology, Cell Biology, Bioengineering, or a related field. 3+ years of relevant industry experience for MS candidates, or 5+ years for BS candidates. Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques. Proficiency in gene editing and cell line development, including protocol optimization and flow-based sorting. Hands-on experience with delivery methods for gene editing tools. Strong communication and interpersonal skills, with the ability to work effectively in a collaborative team environment. Excellent organizational skills and attention to detail in record keeping. Experience in pancreatic islet directed differentiation is highly desirable. Experience in transitioning research-grade processes to GMP-grade is highly desirable.
We are seeking a highly skilled, passionate, and motivated scientist to join our diabetes research team. This position will play a key lab-based role in advancing iPSC stem cell engineering and contribute to the development of the next generation of islet cell therapies.
Responsibilities :
- Enable and advance iPSC gene editing, cell line development, and evaluation of differentiated cells.
- Perform routine iPSC cell culture, passaging, and media preparation.
- Optimize workflows for iPSC CRISPR-based gene editing, gRNA screening, and delivery.
- Develop and perform assays for on- / off-target analysis using PCR and NGS workflows.
- Generate clonal gene-edited iPSC lines and characterize them via flow cytometry, ddPCR, dPCR, and qPCR.
- Culture and execute routine directed differentiation of human iPSCs to pancreatic islets, performing assessments such as cell counts, viability, and metabolite analysis.
- Assist scientists in evaluating and designing mitigation strategies to ensure genomic stability of iPSC lines and drug substances.
- Collaborate cross-functionally to support the transfer of R&D assays to GMP environments.
- Maintain accurate and detailed experimental records, synthesize results, prepare reports, and present findings in meetings.
- Contribute to the development and refinement of SOPs.
- Execute work plans efficiently and meet established deadlines.
Qualifications :
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Biologist • Boston, MA, United States
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