Open Rank Laboratory Sciences Professional Pediatric Cardiology-33276University Staff
Description
University of Colorado Anschutz Medical Campus
Department : Pediatrics - Cardiology
Job Title : Open Rank Laboratory Sciences Professional Pediatric Cardiology
Position #00798209 Requisition #33276
Job Summary :
The Garcia Lab in Pediatric Cardiology is seeking a Laboratory Sciences Professional who is highly motivated and eager to learn, conduct research, and work as a team with other laboratory staff.
The lab studies cellular mechanisms of pediatric heart disease progression with an emphasis on cell metabolism, inflammatory signaling, and lipid synthesis we utilize both human patient samples as well as animal (mouse / rat) models to identify unique adaptations associated with pediatric heart failure to identify novel therapeutic targets.
This Laboratory Sciences Professional will have a key role in conducting a variety of wet lab experiments exploring the molecular mechanisms associated with the progression to heart failure in the pediatric congenital heart disease population.
Candidates should show significant interest in working with murine models.
Opportunities and Training :
- Will work on projects studying mechanisms of heart failure in congenital heart disease.
- Will learn a variety of wet-lab experiments, including several molecular biology techniques, high-resolution mitochondrial respirometry, cardiomyocyte isolation and culture, immune cell isolation and culture, and assays of inflammation.
- Will develop proficiency in collecting, recording, analyzing, and presenting data.
- Will learn mouse / rat IP and SQ injections.
- May assist in the training of new team members on laboratory protocols and operations.
- May assist the principal investigator with the development and writing of research protocols.
- May assist the Principal Investigator with the development and writing of manuscripts and presentations, including as a credited author where appropriate.
Key Responsibilities :
- Processing human whole blood samples, including isolation of peripheral blood mononuclear cells, plasma, and serum.
- In vitro murine primary cardiomyocyte isolation and culture.
- In vivo murine animal model work (mouse / rat), including injections, dissections, survival surgeries, and termination procedures.
- Functional mitochondrial bioenergetics analysis.
- Organization and cataloging of human samples, antibodies, and chemicals.
- Laboratory supply ordering and invoice tracking.
- RNA, DNA, and protein extraction.
- Reagent preparation.
- Immunological assays.
- Protein and RNA quantification (Western blotting, proteomics, RT-qPCR, RNAseq).
- ELISA and enzymatic activity assays.
- Data analysis and presentation.
Work Location :
Onsite this role is expected to work onsite and is located in Aurora, CO.
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