Principal Scientist

Principal Scientist, Protein Production & Process Development

Priority Must Haves :

• Refolded E. coli experience (Ideal)
• If no E. Coli experience, must have
• Experience w / Recombinant protein
• Experience with bispecific protein products
• Strong process development background
• Tech Transfer experience - working with CDMO
• Strong Communication and Leadership experience. Must have previous management experience and ability to speak to management style

NOTES :

• Candidates have fallen short due to lack of E Coli / Recombinant Protein, Bispecific experience
• Mammalian background is not enough
• This is a new role as they work to move into the clinic, building out tech transfer to CDMO to scale process
• Bispecifics and ecoli refolding - E Coli refolding is unique, most haven't done it sense academia.
• This person will be leading a team performing E.Coli refolding
• Strong leadership and communication skills needed
• Bispecific / Trispecific experience is good
• Team size - 3 FTE's, Sci;
• Management experience - Tell me about a time direct report wasn't performing and you needed to step in
• Really positive, leave ego at the door, help colleagues
• Management loves large pharma, believes that to be training ground; but makes for tough transition - small company experience ideal
• Protein biologics,
• E coli refolding
• Bispecifics / recombinant protein
• few years will suffice
• Open to NE relocation
• downstream - Sci 1 that manages 2 other individuals,
• Building out CMC model, hiring VP.
• This person will report into VP CMC, which they're currently hiring for. JOanna Swain, SVP Protein Eng, is the HM on this role now
• Currently securing CDMO
• PhD required
• Not looking for jumpy, Very sensitive to this, technical knowledge, polish - ability articulate the science and story;
• Flagship Pioneering benefits;
• no 401k match right now; offer equity - great time to join

Location : Cambridge, MA

Salary : around $180K

Role Overview

We are seeking an experienced Protein Production and Process Development leader with experience producing protein lead and Development Candidate molecules, preferably with both mammalian and E.

coli production processes. The Principal Scientist will engage a diverse team of research associates and scientists focused on production of lead TCR bispecific molecules and optimization of processes.

The selected candidate and his / her / their team will work in a highly dynamic and cross-functional environment to facilitate seamless technology transfer to an external contract development and manufacturing organization (CDMO).

The incumbent will also enable Repertoire’s proprietary platform technologies with high quality pMHC products produced by microbial or mammalian systems.

The incumbent will interface with internal preclinical Immunology, Protein Modality, and DECODE platform teams, as well as the internal CMC Leader, to provide protein reagents and protein leads, including data packages supporting Development Candidate nomination.

Key Responsibilities

Develop and optimize upstream and downstream processes to generate lead and Development Candidate TCR bispecific and to enable seamless technology transfer to contract manufacturing organizations.

Provide data packages, expertise, and guidance to TCR bispecific candidate nomination decision.

Transfer analytical methods to external CROs / CDMOs and provide additional training, troubleshooting, or technical support, as necessary.

Manage technology development and production of novel pMHC reagents to enable target identification, target validation and TCR affinity maturation for both oncology and / or autoimmune indications.

Drive team engagement, ownership, and agility to deliver ambitious results.

Author and review technical documents and reports for inclusion in CMC sections of regulatory filings.

Qualifications / Experience

• Academic / PhD degree in Biochemistry, Biology, Bioengineering, Immunology, or related discipline.
• Proven experience leading and managing lab teams within complex programs to drive functional and corporate goals.
• Minimum 8+ years’ experience in pharma / biotech industry with management experience in process development.
• Production experience with proteins expressed and refolded from E. coli and with proteins expressed in mammalian cells with oversight of both upstream and downstream process development.
• Experience in research strategy development and operational management of research outcomes through cross-functional and outsourced teams, as well as budget planning and management.
• Technical report writing for inclusion in regulatory submissions.
• Desirable to have experience with bispecific protein products.
• Ability to drive strategic discussions with senior level management and external partners.
• Experience in building practical creativity in teams to persist and respond positively to changes.
• Experience in collaborating across teams to resolve problems with strategic and operational impact on individual, function, and corporate performance.
30+ days ago